All specimens delivered to the laboratory must adhere to the following requirements. Specimens will be accepted only from physicians or authorized clinicians.
Requisition forms must include:
Patient's legal name -- legibly printed or typed
Patient's date of birth
Type of specimen submitted
Office or hospital submitting specimen
Date of service
The specimen container must indicate two unique identifiers:
Patient Name, D.O.B., Social Security number; Medical Record Number
or Type of specimen submitted
All non-gyn specimens must be submitted in a leak-proof container that is placed in a sealed biohazard specimen bag
All bagged specimens shall have an accurately completed requisition accompanying them
Allow all spray fixed and air dried slides to dry completely before placing in cardboard slide carriers for transport
When sending slides fixed in 95% alcohol filled bottles, be certain the caps are tightened before transport
A specimen may be considered unacceptable if certain of the above criteria are not fulfilled. Unlabeled specimens may be returned for proper identification (This is an accreditation requirement)
Note: if surgical specimens are co-submitted--they must be placed in a separate bag from the cytology specimen to avoid formalin fume contamination.
Cytology At-A-Glance Submission Requirements
|Chlamydia smears||One air dried slide|
|Smear for detection of viral changes (Tzanck smear)||Spray fixed slide(s)|
|Pap smear, liquid based-See instructions below||SurePath vial Note: HPV, Chlamydia GC, and HSV tests may be run from the same vial.|
|Pap smear, conventional||Spray fixed slide(s)|
|Smear for maturation index||Spray fixed slide(s)|
|Buccal smear||Spray fixed slide(s)|
|Nipple secretion||Spray fixed slide(s)|
|Smear for detection of viral changes||Spray fixed slide(s)|
|Urethral wash||Add equal amount of cytology fixative, refrigerate|
|Ureteral wash||Add equal amount of cytology fixative, refrigerate|
|Bladder wash||Add equal amount of cytology fixative, refrigerate|
|Urine; voided||Add equal amount of cytology fixative, refrigerate|
|Urine; catheterized||Add equal amount of cytology fixative, refrigerate|
|Bronchial wash||Refrigerate until pickup/delivery; fresh specimen|
|Bronchial brush||Prepared slides; spray fix OR drop into bottle of 95% alcohol|
|Used brush||Place brushes into a container of cytology fixative|
|Sputum||Collect in cytology fixative|
|Cytology and/or Special Stains||Collect in cytology fixative, refrigerate|
|FNA (Fine Needle Aspiration)|
|Prepared smears||Spray fix; air dried slides may be made on some samples|
|Fluids||Express material into a container of cytology fixative|
|Esophageal wash/brush||Refer to bronchial wash/brush instructions above|
|Body Cavity Fluids||Refrigerate until pickup/delivery|
Refer to body cavity fluids
Refer to body cavity fluids
SurePath Submission Instructions
Observe universal precautions for collecting and handling specimens.
Insert the broom into the endocervical canal. Apply gentle pressure until the bristles form against the cervix. Maintaining gentle pressure, hold the stem between the thumb and forefinger. Rotate the brush five times (360 degrees) in a clockwise direction.
Preserve the entire sample by placing your thumb against the back of the broom pad, and disconnecting the broom head from the stem directly into the SurePath preservative vial.
Place the cap on the labeled vial.
Mark the lab requisition with the tests to be performed on the specimen:
a. Pap: Pap smear results
b. HPV: HPV testing performed whether or not the Pap result is abnormal
c. HPV Reflex: HPV testing performed if the Pap result is atypical
d. CT: Chlamydia testing
e. Gonorrhea: Gonorrhea testing
Quad screen: Pap, Reflex HPV, Chlamydia, and Gonorrhea testing
Quad screen plus: Includes the Quad screen and HSV testing.
Note: All of the above tests may be performed on one specimen
Place the vial and requisition into specimen bag and submit to the laboratory.